Genome-large unmarried nucleotide polymorphism (SNP) markers coupled with allele serving recommendations possess emerged just like the a robust unit to own studying state-of-the-art qualities within the cultivated autotetraploid potato (Solanum tuberosum L., 2n = 4? = 48). Yet, this process has been effectively used on the fresh new identity from decimal trait loci (QTLs) root highly heritable faculties such as disease resistance, but mostly unexplored for qualities that have advanced habits regarding inheritance.
Overall performance
In this study, an Fstep 1 tetraploid russet mapping population (162 individuals) was evaluated for multiple quantitative traits over two years and two locations to identify QTLs associated with tuber sugar concentration, processing quality, vine maturity, and other high-value agronomic traits. We report the linkage maps for the 12 potato chromosomes and the QTL location with corresponding genetic models and candidate SNPs explaining the highest phenotypic variation for tuber quality and maturity related traits. Significant QTLs for tuber glucose concentration and tuber fry color were detected on chromosomes 4, 5, 6, 10, and 11. Collectively, these QTLs explained between 24 and 46% of the total phenotypic variation for tuber glucose and fry color, respectively. The QTL on chromosome 10 was associated with apoplastic invertases, with ‘Premier Russet’ contributing the favorable allele for fry processing quality. On chromosome 5, minor-effect QTLs for tuber glucose concentration and fry color co-localized with various major-effect QTLs, including vine maturity, growth habit, tuber shape, early blight (Altenaria tenuis), and Verticillium wilt (Verticillium spp.).
Findings
Linkage data and you may QTL mapping when you look at the an effective russet mapping population (A05141) playing with SNP dose suggestions efficiently known favorable alleles and applicant SNPs for resistance to the new buildup away from tuber reducing glucose. This type of unique markers keeps a high prospect of the advance away from tuber processing top quality. Also, the fresh new breakthrough various genetic activities to own attributes which have overlapping QTLs at maturity locus clearly indicates a different genetic manage.
Background
Having a complicated polyploid genetics this new developed autotetraploid potato (Solanum tuberosum L., 2n = 4? = 48) gift ideas novel pressures for linkage mapping and you can quantitative feature loci (QTL) study. The fresh new latest growth of potato unmarried nucleotide polymorphism (SNP) arrays brings a couple of genome-greater markers which you can use within the mapping and you will quantitative attribute locus https://datingranking.net/escort-directory/beaumont/ (QTL) study [1,dos,step 3,4]bined toward way to obtain analytical products that allow the utilization of allele dosage pointers (i.e., just how many duplicates of any allele during the a given polymorphic locus), it’s now you are able to to somewhat boost the energy out-of SNP information so you’re able to choose recombination events and to use this informative article to the QTL mapping [5,6,7]. At this point, this process might have been properly familiar with place novel QTLs and you will candidate SNPs of this later blight resistance [4, 8], interior temperature necrosis , Potato trojan Y , and agronomic traits , however, largely unexplored having understanding control top quality or any other higher-really worth qualities which have state-of-the-art heredity.
You will find an importance of unit indicators related to tuber running top quality faculties to simply help your selection of germplasm having cultivar innovation. Handling high quality is actually influenced by tuber sugar blogs. The latest buildup out of fructose and you can glucose (reducing sugar) for the potato tubers throughout cold storage negatively influences the conclusion-play with high quality features. Cutting glucose stuff is a complicated attribute controlled by multiple hereditary and you will ecological items [several, 13]. The brand new advanced genetic buildings on the characteristic is actually consistent with the pastime of numerous enzymes active in the metabolic pathways hooking up starch dysfunction and synthesis so you’re able to glucose development and usage [14, 15]. Hereditary mapping research shows you to fry control high quality and you can reducing sugar is actually on the a large number of quick effect QTLs [sixteen,17,18,19,20]. Soltis-Kalina ainsi que al. recognized a couple big QTLs to have processor chip color to your chromosomes 1 and you may six inside an excellent diploid mapping populace. Now, Braun mais aussi al. said a few QTLs for effectiveness cool-caused sweetening to your chromosomes cuatro and you will 6 away from diploid potato. A few of these studies have already been did toward diploid potato germplasm. But not, after that research is necessary to most useful see the hereditary architecture off these types of traits from the tetraploid level in the communities derived from russet-skinned potato types.